Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chiral Polyaniline Helical Nanotubes Immobilized with Pd Nanoparticles for Enantioselective Catalysis.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

An MMP-independent fluorescent probe for simultaneously detecting viscosity and hydrogen peroxide in tumor tissues of cancer patients.

Journal of photochemistry and photobiology. B, Biology·2026

Same author

Alleviation of Hepatotoxicity Induced by Chlorantraniliprole in Zebrafish: A Novel Strategy Based on Carboxymethyl Chitosan-Coated Nanopesticides.

Journal of agricultural and food chemistry·2026

Same author

Chiral Silver-Platinum Hollow Nanostructures: Seedless Synthetic Strategy and Electrochemical Enantioselective Detection.

Inorganic chemistry·2026

Same author

The mediating role of financial behavior in the relationship between psychological capital and financial wellbeing: evidence from a cross-sectional study in China.

Frontiers in psychology·2026

Same author

Functional characterization of gibberellin in controlling flowering time in loquat (Eriobotrya japonica) with autumn and spring flowering habits.

Plant physiology and biochemistry : PPB·2026

Same journal

Recent progress in catalytic asymmetric synthesis of triarylmethanes.

Chemical science·2026

Same journal

GFP chromophore photophysics: ultrafast dynamics and hot ground state cooling in the neutral form.

Chemical science·2026

Same journal

Large Stokes shift fluorophores from <i>meta</i>-substituted zwitterions.

Chemical science·2026

Same journal

<i>In situ</i> glycosylation-directed H-aggregation of Type I photosensitizers for synergistic biofilm eradication and promoting diabetic wound healing.

Chemical science·2026

Same journal

Substituent engineering of dynamic covalent bonds enables simultaneous enhancement of performance and recyclability.

Chemical science·2026

Same journal

Visible-light-enabled three-component carboamidation of alkenes with aryl thianthrenium salts.

Chemical science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 17, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

Multi-level macrocyclic assembly achieving organic upconversion delayed fluorescence for targeted cell imaging.

Jie Niu¹, Xuan Wu², Jie Yu³

¹Medical College, Department of Basic Medicine and Medical Technology, Yangzhou University Yangzhou Jiangsu 225009 China.

Chemical Science

|September 22, 2025

Summary

This summary is machine-generated.

Researchers developed a novel supramolecular assembly for enhanced deep-red luminescence. This purely organic upconversion phosphor system, utilizing macrocyclic confinement, shows promise for advanced targeted cell imaging applications.

More Related Videos

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Jan 17, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Supramolecular Chemistry
Organic Materials Science
Bioimaging Technologies

Background:

Purely organic room-temperature phosphorescence (PORTP) materials are valuable for bioimaging.
Existing PORTP materials often have short excitation wavelengths, limiting their applications.
Developing long-lived, deep-red emitting organic materials is crucial for advanced imaging.

Purpose of the Study:

To engineer a novel purely organic phosphorescence resonance energy transfer (PRET) assembly.
To achieve enhanced PORTP and multi-photon-mediated PRET for delayed deep-red luminescence.
To demonstrate the utility of this assembly for targeted cell imaging.

Main Methods:

Construction of a supramolecular assembly using 6-bromoisoquinolium-modified permethylated β-cyclodextrin (BQ-PCD), cucurbit[8]uril (CB[8]), and tetra(4-sulfonatophenyl)porphyrin (TPPS).
Utilizing macrocyclic confinement (CB[8]) to enhance PORTP lifetime.
Employing multi-photon excitation and phosphorescence resonance energy transfer (PRET) for deep-red emission.
Assembling with hyaluronic acid (HA) for tumor-targeting capabilities.

Main Results:

The BQ-PCD⊂CB[8] assembly significantly prolonged PORTP lifetime (13.8 ns to 640 μs).
The TPPS⊂BQ-PCD⊂CB[8] system achieved efficient PRET (94.5%) yielding delayed deep-red luminescence (around 11 μs lifetime).
Multi-photon excitation at 920 nm enabled deep-red emission via the upconversion process.
The HA-conjugated nanoparticle demonstrated successful targeted cell imaging.

Conclusions:

Macrocyclic confinement effectively enhances PORTP and enables efficient PRET.
The developed upconversion supramolecular nanoparticle provides a novel platform for long-lived, deep-red emission.
This approach offers a promising strategy for advanced targeted cell imaging.