Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chiral Polyaniline Helical Nanotubes Immobilized with Pd Nanoparticles for Enantioselective Catalysis.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

An MMP-independent fluorescent probe for simultaneously detecting viscosity and hydrogen peroxide in tumor tissues of cancer patients.

Journal of photochemistry and photobiology. B, Biology·2026
Same author

Alleviation of Hepatotoxicity Induced by Chlorantraniliprole in Zebrafish: A Novel Strategy Based on Carboxymethyl Chitosan-Coated Nanopesticides.

Journal of agricultural and food chemistry·2026
Same author

Chiral Silver-Platinum Hollow Nanostructures: Seedless Synthetic Strategy and Electrochemical Enantioselective Detection.

Inorganic chemistry·2026
Same author

The mediating role of financial behavior in the relationship between psychological capital and financial wellbeing: evidence from a cross-sectional study in China.

Frontiers in psychology·2026
Same author

Functional characterization of gibberellin in controlling flowering time in loquat (Eriobotrya japonica) with autumn and spring flowering habits.

Plant physiology and biochemistry : PPB·2026
Same journal

Enhanced and selective oxygen reduction by iron porphyrin with a biguanide residue in the second coordination sphere.

Chemical science·2026
Same journal

Excited-state orbital angular momentum enables all-optical molecular spin coherence.

Chemical science·2026
Same journal

Polyvinyl-based hole-transporting materials processed with non-destructive and green solvents for tin-lead perovskite solar cells and all-perovskite tandems.

Chemical science·2026
Same journal

Pd-catalyzed regio- and enantioselective allylation of cyclic allylboronates.

Chemical science·2026
Same journal

Covalent polyoxometalate-polyimide hybridization: multi-scale molecular engineering toward high-performance sodium-ion battery anodes.

Chemical science·2026
Same journal

Catalytic visible light-driven alkane dehydrogenation by a di-uranyl germanotungstate.

Chemical science·2026
See all related articles

Related Experiment Video

Updated: Jan 17, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.3K

Multi-level macrocyclic assembly achieving organic upconversion delayed fluorescence for targeted cell imaging.

Jie Niu1, Xuan Wu2, Jie Yu3

  • 1Medical College, Department of Basic Medicine and Medical Technology, Yangzhou University Yangzhou Jiangsu 225009 China.

Chemical Science
|September 22, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel supramolecular assembly for enhanced deep-red luminescence. This purely organic upconversion phosphor system, utilizing macrocyclic confinement, shows promise for advanced targeted cell imaging applications.

More Related Videos

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.8K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Related Experiment Videos

Last Updated: Jan 17, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.3K
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
11:20

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

7.8K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.3K

Area of Science:

  • Supramolecular Chemistry
  • Organic Materials Science
  • Bioimaging Technologies

Background:

  • Purely organic room-temperature phosphorescence (PORTP) materials are valuable for bioimaging.
  • Existing PORTP materials often have short excitation wavelengths, limiting their applications.
  • Developing long-lived, deep-red emitting organic materials is crucial for advanced imaging.

Purpose of the Study:

  • To engineer a novel purely organic phosphorescence resonance energy transfer (PRET) assembly.
  • To achieve enhanced PORTP and multi-photon-mediated PRET for delayed deep-red luminescence.
  • To demonstrate the utility of this assembly for targeted cell imaging.

Main Methods:

  • Construction of a supramolecular assembly using 6-bromoisoquinolium-modified permethylated β-cyclodextrin (BQ-PCD), cucurbit[8]uril (CB[8]), and tetra(4-sulfonatophenyl)porphyrin (TPPS).
  • Utilizing macrocyclic confinement (CB[8]) to enhance PORTP lifetime.
  • Employing multi-photon excitation and phosphorescence resonance energy transfer (PRET) for deep-red emission.
  • Assembling with hyaluronic acid (HA) for tumor-targeting capabilities.

Main Results:

  • The BQ-PCD⊂CB[8] assembly significantly prolonged PORTP lifetime (13.8 ns to 640 μs).
  • The TPPS⊂BQ-PCD⊂CB[8] system achieved efficient PRET (94.5%) yielding delayed deep-red luminescence (around 11 μs lifetime).
  • Multi-photon excitation at 920 nm enabled deep-red emission via the upconversion process.
  • The HA-conjugated nanoparticle demonstrated successful targeted cell imaging.

Conclusions:

  • Macrocyclic confinement effectively enhances PORTP and enables efficient PRET.
  • The developed upconversion supramolecular nanoparticle provides a novel platform for long-lived, deep-red emission.
  • This approach offers a promising strategy for advanced targeted cell imaging.