Lanthanide(III)-binding peptides and proteins: coordination properties and applications.

Area of Science:

• Biochemistry and Biophysics
• Bioinorganic Chemistry
• Protein Engineering

Background:

• Lanthanides (Ln3+ ions) are vital in medicine, technology, and bacterial metabolism.
• Understanding lanthanide-protein interactions is key for developing new applications.
• Research in lanthanide-binding peptides and proteins is a rapidly advancing field.

Purpose of the Study:

• To provide a comprehensive review of lanthanide-binding peptides and proteins.
• To analyze structural, thermodynamic, and kinetic parameters of Ln3+ interactions.
• To offer guidelines for designing Ln-binding peptides for specific applications.

Main Methods:

• Literature review and critical analysis of existing studies.
• Focus on structural, thermodynamic (affinity, selectivity), and kinetic data.
• Examination of both natural and de novo-designed peptide/protein scaffolds.

Main Results:

• Detailed overview of Ln3+ binding characteristics with various peptides and proteins.
• Identification of key factors influencing binding affinity and selectivity.
• Summary of current applications and design principles.

Conclusions:

• Rational design of Ln-binding peptides/proteins requires understanding interaction parameters.
• Future directions include optimizing peptides for luminescent sensing, MRI, and catalysis.
• This review serves as a guide for advancing lanthanide-biomolecule research.