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Updated: Jun 22, 2026

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation

Published on: September 11, 2012

Modeling Postoperative Pathologic Ileus in Mice: A Simplified and Translational Approach.

Romain Gauthier¹, Julie Thevenin¹, Thibault Planchamp^1,2

¹IRSD, University of Toulouse, INSERM, INRAE, ENVT, Université Toulouse III-Paul Sabatier (UPS), Toulouse, France.

Neurogastroenterology and Motility

|September 22, 2025

Summary

A new mouse model for postoperative ileus (POI) was developed using simple intestinal manipulation, mimicking human surgery. This reproducible model accurately reflects key aspects of human POI, aiding future research into this common complication.

Keywords:

chirurgical procedure gut motility inflammation intestinal transit postoperative ileus

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Last Updated: Jun 22, 2026

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation

Published on: September 11, 2012

A Mouse Model of Intestinal Partial Obstruction

A Mouse Model of Intestinal Partial Obstruction

Published on: March 5, 2018

Postoperative Ileus Murine Model

Postoperative Ileus Murine Model

Published on: July 12, 2024

Area of Science:

Gastroenterology
Surgical Research
Animal Models

Background:

Postoperative ileus (POI) is a common complication following abdominal surgery, characterized by impaired gastrointestinal motility.
Current animal models do not adequately replicate POI associated with digestive sutures or anastomoses.
Surgical trauma, especially during intestinal procedures, significantly contributes to POI severity and prolonged hospitalization.

Purpose of the Study:

To develop a simple, reproducible mouse model of postoperative ileus (POI).
To mimic clinical surgical practices, including intestinal manipulation and anastomosis.
To validate the model's ability to reproduce key pathological features of human POI.

Main Methods:

Mice underwent midline laparotomy and externalization/manipulation of the small intestine for 10 minutes.
An ileo-ileal anastomosis was performed to simulate human surgical interventions.
Gastrointestinal transit, gastric emptying, gut motility, and postoperative inflammation were assessed.

Main Results:

Intestinal manipulation alone induced a pathological POI, comparable to ileo-ileal anastomosis.
The model demonstrated reduced gastric emptying and ileal contractility.
Inflammatory cell infiltration (neutrophils, monocytes/macrophages) was observed in the intestinal muscularis.

Conclusions:

A simple, equipment-free procedure effectively induces POI in mice.
The model accurately mimics clinical surgical actions and reproduces human POI characteristics.
This validated model offers a valuable tool for studying POI pathogenesis and treatment.