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A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
Published on: October 7, 2013
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A Composite Membrane of Decellularized Small Intestinal Submucosa and Polycaprolactone for Bladder Tissue Engineering
Linran Song1, Jingjia Ye1, Xinrang Zhai1,2
1Center for Regeneration and Aging Medicine, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, Zhejiang 322000, China.
ACS Applied Bio Materials
|September 22, 2025
Summary
A new waterproof membrane made from decellularized small intestinal submucosa (dSIS) and polycaprolactone (PCL) shows promise for bladder tissue engineering. This biomaterial composite is biocompatible and suitable for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Urology
Background:
- Autologous intestine grafts for bladder augmentation present significant complications.
- Tissue engineering using biomaterials offers a promising alternative to autologous tissues, mitigating associated risks.
- Decellularized small intestinal submucosa (dSIS) is a bioactive scaffold but lacks waterproof properties essential for urinary applications.
Purpose of the Study:
- To develop a waterproof composite membrane for bladder tissue engineering by combining dSIS with polycaprolactone (PCL).
- To evaluate the biocompatibility and suitability of the dSIS-PCL composite for regenerative medicine in urological applications.
Main Methods:
- A composite membrane was fabricated by combining bioactive dSIS with a biocompatible polymer, polycaprolactone (PCL).
- PCL content was optimized to achieve waterproof properties while preserving dSIS bioactivity.
- The composite membrane's waterproofness, suturability, and biocompatibility were assessed through in vitro cell culture studies and in vivo subcutaneous implantation.
Main Results:
- The dSIS-PCL composite membrane effectively blocked water permeation and was suitable for suturing with a 4-0 suture.
- In vitro biocompatibility tests demonstrated no adverse effects on uroepithelial cells, fibroblasts, or muscle cells regarding viability, proliferation, migration, or mRNA transcription.
- In vivo subcutaneous implantation revealed a minimal inflammatory response, indicating good biocompatibility.
Conclusions:
- The dSIS-PCL composite membrane is a waterproof, biocompatible, and suturable material.
- This composite material holds significant potential as a candidate for bladder tissue engineering applications.
- The developed biomaterial offers a viable alternative to current methods, addressing limitations in bladder augmentation.
