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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

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Triple-signal miRNA detection: A universal biosensor platform based on element labeling and CHA amplification

Xue-Wei Zhang¹, Yuan-Yuan Li², Zhi-Wei Wu²

¹Department of Chemistry, College of Sciences, Beihua University, Jinlin, 132013, China.

|September 22, 2025

Summary

A novel biosensor platform uses element tagging and catalytic hairpin assembly for highly sensitive microRNA (miRNA) detection. This method achieves ultra-low detection limits for improved clinical miRNA assays.

Keywords:

CHA amplification Fluorescence spectroscopy ICP-MS Triple-signal detection miRNA

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Biochemistry
Analytical Chemistry
Nanotechnology

Background:

MicroRNAs (miRNAs) are implicated in diseases like cancer and neurodegeneration.
Current miRNA detection methods lack sensitivity, specificity, and interference resistance.
Development of advanced detection platforms is crucial for clinical applications.

Purpose of the Study:

To develop a universal biosensor platform for highly sensitive and reliable miRNA detection.
To overcome limitations of existing miRNA detection technologies.
To enable precise quantification of miRNAs for potential clinical use.

Main Methods:

Utilized element tagging with Yttrium (Y) in upconversion nanoparticles (UCNPs).
Employed catalytic hairpin assembly (CHA) for signal amplification and target recycling.
Integrated inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence spectroscopy for dual-mode detection.

Main Results:

Achieved ultra-low detection limits: 0.24 fM (ICP-MS), 3.8 fM (fluorescence), and 0.12 fM (SP-ICP-MS).
Demonstrated high sensitivity and specificity through dual-mode signal cross-validation.
Showcased versatility by adapting the platform to various miRNA targets via DNA sequence modification.

Conclusions:

The developed biosensor platform offers a promising solution for sensitive and reliable miRNA detection.
The dual-mode detection and amplification strategy enhance analytical accuracy.
This platform holds potential for advancing clinical miRNA diagnostics.