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Related Concept Videos

Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

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In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
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Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

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In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
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Related Experiment Video

Updated: Jan 17, 2026

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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A Translational Model for In Vivo Intestinal Permeability Assessment in Pigs.

Evelien Van Rymenant1, Christof Van Poucke1, Koen Van Loo1

  • 1Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Merelbeke-Melle, Belgium.

The Journal of Nutrition
|September 22, 2025
PubMed
Summary

This study optimized methods to assess intestinal permeability in pigs, a valuable model for human health research. Findings provide a framework for future studies on diet and gut health.

Keywords:
dextran sodium sulfateindomethacinintestinal inflammationintestinal permeabilitypig

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Area of Science:

  • Gastroenterology
  • Animal Models
  • Nutritional Science

Background:

  • Increased intestinal permeability is linked to noncommunicable diseases and influenced by diet.
  • Pigs serve as relevant translational models for gastrointestinal research.
  • Optimized assessment of intestinal permeability in pigs is crucial for advancing nutritional and health studies.

Purpose of the Study:

  • To develop and refine protocols for inducing and quantifying intestinal permeability in pigs.
  • To establish a reliable translational model for studying diet-induced gut health changes.

Main Methods:

  • Pigs were treated with indomethacin or dextran sodium sulfate (DSS) to induce changes in intestinal health.
  • Intestinal permeability was assessed using urinary recovery of orally administered probes (sucralose, erythritol, Co-EDTA).
  • Fecal inflammatory biomarkers, consistency, body weight, and intestinal tissue morphology were analyzed.

Main Results:

  • Indomethacin significantly increased intestinal permeability, indicated by higher sucralose and Co-EDTA excretion.
  • Indomethacin treatment led to increased fecal inflammatory markers and reduced duodenal villi width.
  • DSS treatment increased fecal inflammatory markers and colon crypt depth but did not alter intestinal permeability.

Conclusions:

  • Developed and optimized a model to disrupt intestinal health in pigs.
  • Provided recommendations for intestinal permeability assessment protocols applicable to both pigs and humans.
  • The established model is suitable for investigating the impact of dietary factors on intestinal permeability and overall gut health.