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Updated: Jan 17, 2026
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Disposable electrochemical aptasensor for rapid and selective vancomycin detection in clinical samples: Bridging
Magdolna Casian1, Oana Hosu-Stancioiu2, Ioana Manea2
1Department of Analytical Chemistry, Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Pasteur Street, 400349, Cluj-Napoca, Romania; Departamento de Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006, Oviedo, Spain; Instituto de Investigación Sanitaria del Principado de Asturias, Av. de Roma s/n, 33011, Oviedo, Spain.
Abstract:
Antibiotic resistance is a growing global threat, with glycopeptides like vancomycin remaining critical for treating resistant infections. However, its narrow therapeutic index necessitates therapeutic drug monitoring (TDM). In this study, we present a disposable electrochemical aptasensor for cost-effective and rapid vancomycin monitoring. Novel high-affinity aptamers were identified via magnetic bead-based SELEX, with their binding confirmed by surface plasmon resonance and computational modeling. The aptasensor, developed on screen-printed gold electrodes using a multipulse-assisted immobilization method, demonstrated a linear response from 2.5 to 50 μM and a detection limit of 1.5 μM. This range encompasses clinically relevant trough (10-13.5 μM) concentrations. Validated in undiluted clinical samples, it showed performance comparable to gold-standard immunoassays, offering a promising tool for vancomycin TDM.
