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Author Spotlight: Understanding Cytokine-Induced Cell Death in Intestinal Epithelial Cells Using Human Organoids
Published on: August 2, 2024
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Visualizing and mapping cell death: Biochemical tools, techniques and assays
Sadhna Soni1, Ashwini Nath Tiwari1, Amrendra Singh1
1Department of Bioscience and Biomedical Engineering, Indian Institute of Technology Bhilai, Chhattisgarh, India.
Progress in Molecular Biology and Translational Science
|September 22, 2025
Summary
Understanding diverse cell death pathways, including apoptosis and newer ones like ferroptosis, is crucial for developing novel cancer therapies. Investigating these processes aids in overcoming treatment resistance and improving patient outcomes.
Area of Science:
- Cellular Biology
- Molecular Biology
- Biochemistry
Background:
- Cell death is vital for multicellular organism development, tissue balance, and immunity.
- Dysregulated cell death contributes to diseases like cancer, where tumor cells often evade apoptosis, causing treatment resistance.
Purpose of the Study:
- To systematically review molecular and cellular changes in various cell death pathways.
- To discuss conventional and non-conventional methods for investigating cell death processes.
- To highlight the therapeutic potential of exploring cell death pathways beyond apoptosis for cancer treatment.
Main Methods:
- Review of existing literature on cell death mechanisms.
- Systematic discussion of biochemical and cellular analysis techniques.
- Comparative analysis of different cell death pathways (apoptosis, necroptosis, pyroptosis, ferroptosis, cuproptosis).
Main Results:
- Multiple cell death pathways exist, each with distinct molecular triggers and outcomes.
- Apoptosis evasion is a common mechanism of cancer treatment resistance.
- Emerging cell death pathways (necroptosis, pyroptosis, ferroptosis, cuproptosis) show promise for novel cancer therapies.
Conclusions:
- A thorough understanding of diverse cell death mechanisms and analytical methods is essential for advancing cancer therapeutics.
- Exploring non-apoptotic cell death pathways offers new avenues for overcoming drug resistance in cancer treatment.
- Selecting appropriate detection and quantification methods is critical for accurate cell death research.
