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Perturbative Fourier ptychographic microscopy for fast quantitative phase imaging.

Martin Zach, Kuan-Chen Shen, Ruiming Cao

    Optics Express
    |September 23, 2025
    PubMed
    Summary
    This summary is machine-generated.

    We developed perturbative Fourier ptychographic microscopy (pFPM), a faster, high-resolution imaging technique. pFPM combines differential phase contrast (DPC) with dark-field illumination for improved quantitative phase imaging.

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    Area of Science:

    • Microscopy
    • Optical Imaging
    • Computational Imaging

    Background:

    • Fourier ptychographic microscopy offers high resolution but slow acquisition.
    • Differential phase contrast (DPC) provides fast imaging but limited resolution.

    Purpose of the Study:

    • Introduce perturbative Fourier ptychographic microscopy (pFPM) for fast, high-resolution, wide-field quantitative phase imaging.
    • Overcome the resolution limitations of conventional DPC.

    Main Methods:

    • Extend DPC using a Gauss-Newton algorithm with regularization for multiple iterations.
    • Incorporate dark-field illumination, specifically tailored ring-shaped patterns.
    • Develop enhanced phase reconstruction algorithms.

    Main Results:

    • Achieve fast, high-resolution, wide-field quantitative phase imaging with fewer measurements.
    • Demonstrate improved reconstruction quality compared to other illumination schemes.
    • Combine enhanced algorithms with specialized illumination for significant advantages.

    Conclusions:

    • Perturbative Fourier ptychographic microscopy (pFPM) offers a breakthrough in imaging speed and resolution.
    • The developed methodology enhances quantitative phase imaging capabilities.
    • pFPM provides a powerful tool for various scientific applications requiring detailed phase information.