Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

20.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

12.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Etiology and pathogens of fungal endophthalmitis].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2015
Same author

Eucommia ulmoides Oliv. bark aqueous extract inhibits osteoarthritis in a rat model of osteoarthritis.

Journal of ethnopharmacology·2015
Same author

Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes.

International immunopharmacology·2015
Same author

Treatment with recombinant lubricin attenuates osteoarthritis by positive feedback loop between articular cartilage and subchondral bone in ovariectomized rats.

Bone·2015
Same author

Tet1-mediated DNA demethylation regulates neuronal cell death induced by oxidative stress.

Scientific reports·2015
Same author

Authors' reply.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2015

Related Experiment Video

Updated: Jan 17, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

Zero-shot learning for denoising and super-resolution in multifocal structured illumination microscopy.

Zizhen Jiang, Fuhong Han, Mengjiao Nie

    Optics Express
    |September 23, 2025
    PubMed
    Summary

    Multifocal structured illumination microscopy (MSIM) offers enhanced 3D super-resolution imaging. A novel Zero-shot Deconvolution Networks (ZSDN) approach improves MSIM image quality, enabling faster, lower-photodamage live-cell and tissue imaging.

    More Related Videos

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
    12:44

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

    Published on: September 29, 2014

    20.4K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K

    Related Experiment Videos

    Last Updated: Jan 17, 2026

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.1K
    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
    12:44

    Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

    Published on: September 29, 2014

    20.4K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.3K

    Area of Science:

    • Biomedical Imaging
    • Microscopy Technology
    • Optical Physics

    Background:

    • Multifocal structured illumination microscopy (MSIM) provides enhanced resolution and depth for 3D super-resolution imaging of biological specimens.
    • Low signal-to-noise ratio (SNR) in raw MSIM data leads to reconstruction artifacts, limiting imaging speed and fidelity.
    • Current limitations hinder MSIM's application in live-cell and tissue super-resolution imaging.

    Purpose of the Study:

    • To develop a novel preprocessing method for MSIM data to overcome SNR limitations.
    • To enhance the quality and speed of MSIM super-resolution reconstruction.
    • To enable low-photodamage in vivo imaging applications using MSIM.

    Main Methods:

    • Implementation of Zero-shot Deconvolution Networks (ZSDN) for denoising and deconvolution of raw MSIM data.
    • Integration of ZSDN preprocessing with pixel reassignment for MSIM super-resolution reconstruction (ZSDN-MSIM).
    • Evaluation of the proposed method under low-illumination conditions.

    Main Results:

    • The ZSDN-MSIM approach significantly improves the imaging quality of MSIM data.
    • Artifacts introduced by low SNR are substantially reduced.
    • Enhanced image fidelity and resolution are achieved, particularly under low-illumination settings.

    Conclusions:

    • ZSDN-MSIM effectively addresses the SNR challenges in MSIM imaging.
    • The methodology facilitates high-quality, low-photodamage super-resolution imaging.
    • This advancement holds significant potential for in vivo live-cell and tissue imaging applications.