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CAMSAP3 Suppresses Breast Cancer Metastasis and Serves as an Independent Prognostic Marker
Chao Han1, Caiping Chen1, Li Xue1
1Department of Breast Surgery, Affiliated Hospital of Jiaxing University (First Hospital of Jiaxing), Jiaxing, China.
Technology in Cancer Research & Treatment
|September 23, 2025
Summary
Calmodulin-regulated spectrin-associated protein 3 (CAMSAP3) acts as a metastasis suppressor in breast cancer. High CAMSAP3 expression correlates with better survival and reduced tumor aggressiveness, indicating its prognostic value.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Breast cancer metastasis is a primary cause of cancer mortality.
- The role of calmodulin-regulated spectrin-associated protein 3 (CAMSAP3) in breast cancer metastasis is not well understood.
- CAMSAP3 is known to suppress metastasis in other cancer types.
Purpose of the Study:
- To investigate the prognostic significance of CAMSAP3 in breast cancer.
- To determine the functional role of CAMSAP3 in breast cancer cell migration and invasion.
Main Methods:
- Immunohistochemistry was used to assess CAMSAP3 protein expression in 137 primary breast cancer specimens.
- Statistical analyses, including Kaplan-Meier and Cox regression, were performed to evaluate distant relapse-free survival (DRFS).
- In vitro functional assays (wound healing, Transwell invasion) and external mRNA dataset validation were conducted.
Main Results:
- High CAMSAP3 expression was linked to favorable clinicopathological features (e.g., lower grade, hormone receptor positivity).
- Elevated CAMSAP3 levels significantly correlated with improved DRFS and distant metastasis-free survival (DMFS).
- CAMSAP3 overexpression suppressed, while knockdown enhanced, cancer cell migration and invasion in vitro.
Conclusions:
- CAMSAP3 functions as a potential metastasis suppressor in breast cancer.
- CAMSAP3 is an independent prognostic marker, with high expression indicating a less aggressive phenotype and better patient outcomes.
- The inhibitory effect on cell migration and invasion may underlie CAMSAP3's metastasis-suppressing role.
