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Related Experiment Video

Updated: Jan 17, 2026

Isolating Intestinal Stem Cells from Adult Drosophila Midguts by FACS to Study Stem Cell Behavior During Aging

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Published on: December 16, 2014

Aging Reduces Intestinal Stem Cell Activity in Killifish and Intermittent Fasting Reverses Intestinal Gene Expression

Michael Kothmayer¹, Sylvia Laffer¹, Philipp Widmayer¹

¹Division of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria.

|September 23, 2025

Summary

Aging impairs intestinal tissue homeostasis and stem cell activity. Intermittent fasting in adulthood can preserve gut structure and function in old age, suggesting potential benefits for longevity.

Keywords:

aging intestinal stem cells killifish nutrition rejuvenation senescence stomach

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Area of Science:

Gerontology
Gastroenterology
Cell Biology

Background:

Aging leads to cellular and organ dysfunction, impacting health and longevity.
Dietary restriction shows promise in counteracting aging effects, but its impact on specific organs requires further investigation.

Purpose of the Study:

To investigate the effects of aging and nutrition on the African turquoise killifish intestine throughout its lifespan.
To understand how intermittent fasting influences gut tissue homeostasis and stem cell activity during aging.

Main Methods:

Utilized the African turquoise killifish as a model organism for aging studies.
Integrated morphological measurements, histology, and transcriptomics to analyze intestinal changes.
Examined gut tissue at various life stages, including neonatal, adolescent, adult, and old age.

Main Results:

Observed age-dependent declines in intestinal length, volume, and stem cell cycle time.
Transcriptomic analysis revealed significant changes in circadian clock and stem cell niche markers with age.
Intermittent fasting in adulthood maintained adult gene expression levels in old age, preserving tissue homeostasis.

Conclusions:

Aging is associated with reduced intestinal stem cell activity and tissue homeostasis.
Intermittent fasting can counteract age-related decline in gut structure and function.
Findings in killifish intestine are translatable to mammalian gut biology and aging research.