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Updated: Jan 17, 2026
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Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
Published on: July 15, 2015
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Metabolite-enhanced normothermic machine perfusion improves kidney transplant viability
Jan Czogalla1,2, Fabian Hausmann2,3, Simon Lagies4,5
1III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
JCI Insight
|September 23, 2025
Summary
Normothermic machine perfusion (NMP) for kidneys faces challenges due to variable solutions. A new metabolite-enhanced solution protected kidney cells, improving transplant outcomes in cross-species studies.
Area of Science:
- Organ transplantation research
- Machine perfusion technologies
- Renal cell biology
Background:
- Normothermic machine perfusion (NMP) expands organ availability for transplantation.
- Current NMP perfusion solutions for kidneys lack standardization, posing challenges.
- Kidney injury during NMP is a significant concern impacting transplant success.
Purpose of the Study:
- To develop a standardized, effective perfusion solution for NMP in kidney transplantation.
- To investigate cellular injury mechanisms associated with NMP in kidneys.
- To improve renal proximal tubular cell viability and transplant outcomes using NMP.
Main Methods:
- Created a multimodal, cross-species cellular atlas of NMP-associated kidney injury.
- Utilized a literature-based consensus buffer to establish a framework.
- Developed and tested a novel metabolite-enhanced perfusion solution.
Main Results:
- The developed cellular atlas provided a systematic framework for solution design.
- The metabolite-enhanced solution demonstrated protective effects on renal proximal tubular cells.
- Improved cellular viability and enhanced transplantation outcomes were observed across species, including human kidneys.
Conclusions:
- A standardized, metabolite-enhanced perfusion solution can mitigate NMP-induced kidney injury.
- This approach improves cellular protection and transplant outcomes.
- The findings support the broader application of NMP for kidney transplantation.
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