Nafion-stabilized silver nanoparticles modified glassy carbon electrode for ultrasensitive detection of alpha-1-acid...

In this study, alpha-1-acid glycoprotein (AGP), a crucial biomarker associated with various diseases and physiological conditions, was selected for detection using the developed immunosensor. The immunosensor was fabricated by depositing a synthesized Nafion/silver nanoparticles (AgNPs) nanocomposite on the glassy carbon electrode as the substrate material. The changes in the physicochemical properties after Nafion/AgNPs nanocomposite deposition were analyzed through SEM and XPS measurements. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to analyze the immunosensor fabrication's electrochemical performance. AGP was detected through the immobilized anti-AGP on the electrode surface. The developed immunosensor exhibited a wide linear detection range from 0.05 to 3.2 mg/mL and a LOD of 0.17 mg/mL. The developed immunosensor showed excellent selectivity and repeatability when subjected to different kinds of interfering proteins. The recovery of AGP ranged from 101.7 to 103.7 % and RSD was <4 %, indicating high accuracy in real samples detection. The immunosensor developed for AGP detection showed a number of beneficial characteristics, including low cost and small volume sample requirements, making it highly suitable for point-of-care applications. This study provides new insights into the precise fabrication and affordable immunosensors for diverse clinical diagnostic applications.