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Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

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The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
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Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
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Vascularizing stem cell-derived islets: A blueprint for functional maturation.

Yan Xiong1, Per-Olof Berggren2

  • 1The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden.

Developmental Cell
|September 23, 2025
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Summary
This summary is machine-generated.

Embedding human pluripotent stem cell-derived islets with endothelial cells and fibroblasts accelerates beta cell maturation. This approach enhances insulin secretion and in vivo graft performance through microvessel formation and extracellular matrix proteins.

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Area of Science:

  • Stem cell biology
  • Endocrinology
  • Vascular biology

Background:

  • Human pluripotent stem cells (hPSCs) are a potential source for beta cell replacement therapy.
  • Maturation of hPSC-derived beta cells remains a challenge for effective diabetes treatment.

Purpose of the Study:

  • To investigate methods for accelerating the functional maturation of hPSC-derived islets.
  • To enhance beta cell function and in vivo performance through co-culture with vascular cells.

Main Methods:

  • Co-culture of hPSC-derived islets with endothelial cells and fibroblasts.
  • Formation of perfusable microvessels within the islet construct.
  • Assessment of beta cell calcium response and insulin secretion.
  • Evaluation of in vivo graft performance in a mouse model.

Main Results:

  • Embedding islets with endothelial cells and fibroblasts accelerated beta cell functional maturation.
  • Formation of perfusable microvessels was observed.
  • Endothelial cell-derived extracellular matrix proteins and BMP2/4 signaling enhanced beta cell calcium response and insulin secretion.
  • Improved in vivo graft performance was achieved in the co-cultured constructs.

Conclusions:

  • Co-culture of hPSC-derived islets with endothelial cells and fibroblasts promotes beta cell maturation and vascularization.
  • This strategy enhances beta cell function and therapeutic potential for diabetes.
  • Extracellular matrix and BMP signaling play key roles in beta cell maturation and function.