Constructing elongated Corynebacterium glutamicum and its application in efficient l-lysine production during...

Enhancing biofilm formation ability is of importance for producing bio-chemicals during biofilm-based continuous fermentation. The elongated cell phenotype has been shown to promote cell attachment and aggregation, which facilitates biofilm formation. Here, an elongated Corynebacterium glutamicum (C. glutamicum) Cg-AsftsZ was constructed by downregulating cell division gene ftsZ, and the l-lysine titer and physiological changes during biofilm-based continuous fermentation were investigated. The results showed that Cg-AsftsZ achieved an average l-lysine titer of 143.3 g/L and a yield of 66.4 % (l-lysine/glucose), which is a 6.8 % improvement compared to the wild-type strain. Physiological analysis showed that the biofilm cells of Cg-AsftsZ maintained high viability and DNA content throughout fermentation, indicating strong metabolic stability. Collectively, these results demonstrated that inhibiting ftsZ expression to construct elongate cells in C. glutamicum can promote biofilm formation and thereby improve l-lysine yield during continuous fermentation, offering a valuable insight for developing biofilm-based fermentation.