3D Printing of genipin crosslinked alginate/gelatin Ink for tissue engineering application

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Tissue engineering requires scaffolds mimicking the extracellular matrix for cell growth and regeneration.
• Developing advanced bioinks is crucial for creating functional tissue constructs.

Purpose of the Study:

• To formulate and characterize a novel 3D-printable bioink from gelatin and alginate, crosslinked with genipin.
• To optimize genipin concentration for enhanced mechanical properties and biological performance in tissue engineering scaffolds.

Main Methods:

• A composite bioink of 7% gelatin and 9% alginate was prepared.
• Genipin crosslinking was performed at varying concentrations (0%, 0.1%, 0.5%, 1%).
• Extrusion-based 3D printing was optimized, followed by physicochemical and biological evaluations.

Main Results:

• Genipin crosslinking improved scaffold compressive modulus and degradation resistance.
• Optimal cell viability and adhesion were observed at 0.1% and 0.5% genipin concentrations.
• Higher genipin concentrations (1%) reduced cell adhesion, viability, and hemocompatibility.

Conclusions:

• Genipin-crosslinked alginate/gelatin hydrogels offer tunable properties for tissue engineering.
• This bioink platform supports the development of functional tissue constructs with balanced mechanical and biological attributes.
• The study demonstrates a promising approach for advanced tissue regeneration applications.