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FET fusion oncoproteins enrich SWI/SNF complex subtypes and interaction partners

Malin Lindén1, Lisa Andersson1, Heba Albatrok1

  • 1Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Center for Cancer Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Cellular & Molecular Biology Letters
|September 23, 2025
PubMed
Abstract

Insights

FET oncoproteins interact with SWI/SNF complexes in sarcomas, influencing their composition and function. These interactions, along with epigenetic changes, contribute to specific tumor types in FET sarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • FET (FUS, EWSR1, TAF15) fusion oncoproteins are key in sarcomas and leukemias.
  • FET oncoproteins interact with SWI/SNF chromatin remodeling complex subtypes.
  • The precise impact on SWI/SNF composition and epigenetic effects is not fully understood.

Purpose of the Study:

  • To investigate how FET oncoproteins affect SWI/SNF complex composition and interactomes.
  • To determine the downstream epigenetic consequences in FET sarcomas.

Main Methods:

  • Utilized immunoprecipitation and quantitative mass spectrometry.
  • Validated complex composition and interactions using glycerol gradient sedimentation and co-immunofluorescence.
  • Analyzed chromatin accessibility (ATAC-seq) and gene regulation (RNA-seq) in FET sarcomas.

Main Results:

  • FET sarcomas exhibit distinct SWI/SNF complex compositions with unique subunit paralogs and interaction partners.
  • FET oncoproteins do not significantly disrupt SWI/SNF complex integrity.
  • FUS::DDIT3-bound SWI/SNF complexes in myxoid liposarcoma are enriched in PBAF and GBAF components.

Conclusions:

  • FET oncoproteins function with assembled, functional SWI/SNF complexes and recruited partners.
  • SWI/SNF composition, interactomes, and epigenetics collectively influence FET sarcoma tumor type.
Keywords:
Ewing sarcomaFET fusion oncoproteinsMyxoid liposarcomaQuantitative mass spectrometrySWI/SNF chromatin remodeling complex

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