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Related Experiment Video

Updated: Jan 17, 2026

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential for Vascularised Tissue Engineering-Laboratory

Shuntaro Yamada1,2, Katerina Holomkova1,3,4, Åshild Johansen1

  • 1Center of Translational Oral Research - Tissue Engineering, Department of Clinical Dentistry, University of Bergen, Bergen, Norway.

International Endodontic Journal
|September 24, 2025
PubMed
Summary

Long-term biobanked human dental pulp stem cells (DPSCs) retain their ability to promote blood vessel growth. These cells can be used to engineer vascularized tissues for regenerative dentistry.

Keywords:
angiogenesisdental pulpdental researchmesenchymal stem cellsregenerative medicinetissue engineering

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Stem Cell Biology

Background:

  • Human dental pulp stem cells (DPSCs) are a promising source for regenerative therapies.
  • Long-term biobanking of stem cells is crucial for clinical applications.
  • Assessing the angiogenic potential of biobanked DPSCs is vital for tissue engineering.

Purpose of the Study:

  • To evaluate the pro-angiogenic properties of DPSCs after 7-8 years of biobanking.
  • To determine if biobanked DPSCs can be used for engineering vascularized tissues.
  • To assess the clinical translation potential of biobanked DPSCs in regenerative dentistry.

Main Methods:

  • Cryopreserved DPSCs were characterized for chromosomal integrity, immunophenotype, and multipotency.
  • In vitro pro-angiogenic conditioning was performed, followed by gene and protein expression analysis.
  • Angiogenic capacity was assessed using tube formation assays, CAM implantation, organ-on-chip models, and 3D hydrogel cultures.

Main Results:

  • Biobanked DPSCs maintained their stem cell identity and differentiation potential.
  • Endothelial conditioning enhanced angiogenic gene expression in DPSCs.
  • Both naive and conditioned DPSCs promoted vascular ingrowth, with conditioned DPSCs generating vascularized tissue in 3D hydrogels.

Conclusions:

  • Long-term biobanked DPSCs retain significant angiogenic potential.
  • DPSCs, particularly after endothelial induction, can independently generate vascularized tissue in vitro.
  • These findings highlight the clinical applicability of biobanked DPSCs for vascularized tissue engineering and regenerative therapies.