Temporal Changes in Tear Film Stability With Four Dry Eye Eyedrops in Healthy Subjects

This study aimed to compare temporal changes in tear meniscus height (TMH) and noninvasive tear film break-up time after the instillation of four dry eye treatments: artificial tears (AT), 3% diquafosol sodium long-acting (DQL), 3% diquafosol sodium (DQS), and rebamipide (RB).

Sixty healthy eyes were randomly divided into four treatment groups. The TMH and noninvasive tear film break-up time were measured using a noninvasive tear film analysis device at baseline and 1, 5, 15, 30, 60, 120, and 180 min after instillation.

TMH and noninvasive tear film break-up time did not show significant differences among the groups. In the AT and RB groups, TMH increased significantly 1 min after instillation compared with baseline. In the DQS group, TMH remained elevated for up to 60 min, whereas the DQL group showed a more prolonged increase, lasting up to 120 min. For the noninvasive tear film break-up time, significant prolongation was observed only at 5 and 15 min after instillation in the DQL group.

Topical instillation of 3% diquafosol ophthalmic solution effectively increased tear volume on the ocular surface, with the long-acting DQL formulation demonstrating a more sustained effect in healthy eyes.