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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Environmental isolation of Sporothrix spp. in veterinary settings
Regielly Caroline Raimundo Cognialli1,2, Carolina Melchior do Prado2, Giovana Maria da Silva3
1Federal University of Paraná, Hospital de Clínicas Curitiba Brazil.
Background:
This study investigated environmental contamination by Sporothrix spp. in a veterinary facility specialised in treating cats with sporotrichosis.
Methods:
Twelve samples were collected from frequently touched surfaces and cages of three cats at different treatment stages. Sampling sites included a procedure table, door handle, faucet, ethanol sprayer, an identification card with visible secretions, and cage components. Samples were cultured on Mycosel agar. Fungal growth was assessed through micromorphological analysis.
Results:
Sporothrix was isolated from four samples (33%), one from the stainless-steel procedure table and the others associated with the cages of two symptomatic cats. Cat #1, with active ulcerative lesions, had positive cultures from both the cage grid and latch. Cat #2, in early treatment and showing respiratory signs, had fungal growth on the cage grid. No contamination was detected from Cat #3's environment, and for the remaining sports swapped.
Conclusion:
These findings suggest that cage surfaces in veterinary settings can be a source of environmental contamination and potential occupational exposure.
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