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Advancing Untargeted Metabolomics Through FT-ICR-MS: Challenges and Applications.

Marta Sousa Silva1, Carlos Cordeiro1

  • 1FT-ICR and Structural Mass Spectrometry Laboratory Biosystems and Integrative Sciences Institute (BioISI) Faculdade De Ciências Universidade De Lisboa Lisbon Portugal.

Analytical Science Advances
|September 24, 2025
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Summary
This summary is machine-generated.

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) offers unparalleled performance for untargeted metabolomic analysis. This review highlights advancements, applications, and future prospects of FT-ICR-MS in understanding complex biological systems.

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Area of Science:

  • Metabolomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Metabolomics studies the complete set of small molecules (metabolites) in biological systems.
  • It captures biochemical pathways and dynamic interactions, reflecting physiological and environmental states.
  • Untargeted metabolomic analysis aims to identify and quantify all detectable metabolites.

Purpose of the Study:

  • To review new developments in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) for metabolomics.
  • To discuss new methodologies and recent applications of FT-ICR-MS in the field.
  • To address current challenges and future perspectives for FT-ICR-MS in metabolomic research.

Main Methods:

  • Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is the primary technology discussed.
  • FT-ICR-MS provides extreme mass resolution and exceptional mass accuracy.
  • This enables precise identification and differentiation of thousands of metabolites in complex samples.

Main Results:

  • FT-ICR-MS allows for the simultaneous detection of thousands of compounds.
  • It provides highly accurate molecular formulas based on exact mass and isotopic distribution.
  • This technology is unmatched by other mass spectrometers for untargeted metabolomics.

Conclusions:

  • FT-ICR-MS is the highest-performance technology for untargeted metabolomic analysis.
  • Advancements in FT-ICR-MS continue to enhance its capabilities in biological research.
  • The technology holds significant promise for future metabolomic studies and applications.