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Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma
A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (gammaG and free kappa chain) and C57BL/6N lymphoma that is resistant to bromo-deoxyuridine and does not produce immunoglobulins. The hybrid cells contained the membrane antigens of both parents; they synthesized free kappa chain; no synthesis of gammaG (gamma(2a)) heavy chain was detected.
A hybrid cell line was established by cell fusion between a cloned Balbic myeloma that is resistant to 8-azaguanine and produces immunoglobulin (gammaG and free kappa chain) and C57BL/6N lymphoma that is resistant to bromo-deoxyuridine and does not produce immunoglobulins. The hybrid cells contained the membrane antigens of both parents; they synthesized free kappa chain; no synthesis of gammaG (gamma(2a)) heavy chain was detected.