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Updated: Jan 17, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Hymenobacter cheonanensis sp. nov., and Hymenobacter convexus sp. nov. isolated from the construction site
Ji-Yun Son1, Sathiyaraj Srinivasan2
1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 01797, Korea.
Abstract:
Two aerobic bacterial isolates, designated as strains CA2-7T and CA1UV-4T, were isolated from soil samples collected at a construction site in Cheonan. Both strains were identified as Gram-stain-negative, rod-shaped and a phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains constitute a distinct lineage within the family Hymenobacter (order Cytophagales, class Cytophagia). The closest genetic relatives were found to be members of the genus Hymenobacter, specifically Hymenobacter ginkgonis HMF4947T (with 16S rRNA gene sequence similarity of 97.28%) and Hymenobacter segetis S7-3-11T (98.10%). Both strains grew optimally at pH 7.0, 25 °C, without NaCl. Fatty acid analysis revealed distinctive profiles, with C15:0 anteiso and C15:0 iso predominating in strain CA2-7T, while C15:0 iso and summed feature 3 (C16:1 ω7c/C16:1 ω6c) were the primary fatty acids in strain CA1UV-4T. Both strains exhibited MK-7 as the major respiratory quinone. Biochemical, chemotaxonomic, and phylogenetic data support CA2-7T and CA1UV-4T as new Hymenobacter species. Accordingly, we suggest the names Hymenobacter cheonanensis and Hymenobacter convexus for strains CA2-7T (= KCTC 92968T = NBRC 116577T) and CA1UV-4T (= KCTC 92970T = NBRC 116576T), respectively.
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