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MiR-148a-3p regulates the fracture healing process by targeting MAFB
Yongheng Li1, Shuwei Yang2, Ganggang Wang3
1Department of Orthopedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, China.
Background:
Osteoporotic vertebral fracture (OVF) is an extremely common fragile fracture in the elderly population.
Aims:
To explore the expression changes, clinical significance and mechanism of action of miR-148a-3p in the postoperative healing process of patients with OVF.
Methods:
185 OVF patients who received percutaneous vertebroplasty (PVP) treatment were selected as the research subjects. RT-qPCR was used to detect the expression levels of miR-148a-3p and genes. ROC curve analysis was applied to evaluate the relationship between miR-148a-3p and postoperative healing, as well as its diagnostic value for delayed healing. In vitro, miR-148a-3p overexpression and knockdown cell models were established to investigate its potential mechanism in fracture healing.
Results:
Serum miR-148a-3p expression in OVF patients was significantly higher than in healthy controls, and it gradually decreased over time after surgery. The expression of miR-148a-3p in the delayed healing group is significantly higher than that in the healing group, and it showed potential diagnostic value for delayed healing (AUC = 0.859). In addition, miR-148a-3p is an independent risk factor for delayed healing. In cell experiments, during the process of osteogenic induction, expression of miR-148a-3p decreased, while the expression of osteogenic markers increased. Mechanistically, miR-148a-3p directly targeted and inhibited MAFB expression; its upregulation suppressed cell proliferation and osteogenic marker expression, and these inhibitory effects were reversed by MAFB overexpression.
Conclusions:
miR-148a-3p inhibits osteoblast proliferation and differentiation by targeting and suppressing MAFB, participates in the post-OVF healing process.
Insights
MicroRNA-148a-3p is elevated in osteoporotic vertebral fractures (OVF) and hinders bone healing by inhibiting MAFB. Lowering miR-148a-3p may improve fracture recovery.
Area of Science:
- Biochemistry
- Molecular Biology
- Orthopedics
Background:
- Osteoporotic vertebral fracture (OVF) is a prevalent fragility fracture in the elderly.
- Understanding molecular mechanisms in OVF healing is crucial for therapeutic development.
Purpose of the Study:
- To investigate the expression, clinical significance, and mechanism of miR-148a-3p in OVF postoperative healing.
- To assess miR-148a-3p's role in delayed fracture healing and its diagnostic potential.
Main Methods:
- Serum miR-148a-3p levels were quantified using RT-qPCR in 185 OVF patients treated with percutaneous vertebroplasty (PVP).
- ROC curve analysis evaluated diagnostic value for delayed healing.
- In vitro cell models examined miR-148a-3p's mechanism in osteogenesis.
Main Results:
- OVF patients showed higher serum miR-148a-3p than controls; levels decreased post-surgery.
- Elevated miR-148a-3p correlated with delayed healing (AUC=0.859) and was an independent risk factor.
- miR-148a-3p targeted and inhibited MAFB, suppressing osteoblast proliferation and differentiation.
Conclusions:
- miR-148a-3p negatively regulates osteoblast function by targeting MAFB.
- This microRNA plays a significant role in the impaired healing process following OVF.
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