Engineered a novel polyoxometalates-based composite platform with pH-responsive selectivity for efficient and...

Area of Science:

• Materials Science
• Biochemistry
• Separation Science

Background:

• Egg white is a complex matrix containing valuable proteins like ovalbumin (Ova) and lysozyme (Lys).
• Efficient and selective separation methods are crucial for utilizing these proteins in functional foods and ensuring food safety.

Purpose of the Study:

• To engineer a novel polyoxometalates composite for the efficient and controllable separation of Ova and Lys from egg white.
• To investigate the pH-responsive selectivity of the composite for protein isolation.

Main Methods:

• Synthesis of a polyoxometalates composite (SbW9@PEI) using a liquid-phase method.
• Utilizing pH-responsive selectivity for adsorption and separation of Ova and Lys.
• Characterization of protein purity and identification of co-isolated low-abundance proteins.

Main Results:

• The SbW9@PEI composite demonstrated high adsorption efficiency for Ova (96%) at pH 4.0 and Lys (100%) at pH 11.0.
• Achieved high adsorption capacities of 266.5 mg/g for Ova and 366.3 mg/g for Lys.
• Successfully isolated high-purity Ova and Lys from egg white, enabling identification of additional low-abundance proteins.

Conclusions:

• The engineered polyoxometalates composite offers an efficient and selective method for separating Ova and Lys from complex biological samples.
• This approach has significant implications for functional food development and food safety analysis.
• The method enhances the detection of low-abundance proteins, providing deeper insights into egg white composition.