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Updated: Jun 27, 2026

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
08:52

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

Published on: August 30, 2017

A Protocol to Assess Time-of-Day-Dependent Learning and Memory in Mice Using the Novel Object Recognition Test.

Jordan Mar1,2, Matthew A McGregor1, Vishnuvasan Raghuraman3

  • 1Department of Biology, Texas A&M University, College Station, TX, USA.

Bio-Protocol
|September 26, 2025
PubMed
Summary

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This summary is machine-generated.

This study presents a novel protocol for the novel object recognition (NOR) test in mice, enabling learning and memory assessment during both active and inactive periods. The method accounts for circadian influences on cognitive performance.

Area of Science:

  • Neuroscience
  • Behavioral Science
  • Chronobiology

Background:

  • Learning and memory are crucial behavioral indicators of brain function.
  • The novel object recognition (NOR) test is a standard method for assessing memory and learning.
  • Circadian rhythms and sleep-wake cycles significantly influence cognitive processes, necessitating research during animals' active periods.

Purpose of the Study:

  • To develop and validate a protocol for performing the NOR task in mice during both their active (dark) and inactive (light) phases.
  • To provide a flexible framework for investigating the impact of time-of-day and activity cycles on learning and memory.
  • To identify critical methodological details for reliable behavioral responses in the NOR test.

Main Methods:

  • Implementation of a standardized NOR task protocol adapted for both light and dark phases in adult mice.
Keywords:
Behavioral assayCircadian rhythms/time of dayLearning and memoryMice behavior protocolNovel object recognition

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Last Updated: Jun 27, 2026

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice
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Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

Published on: August 30, 2017

Novel Object Recognition and Object Location Behavioral Testing in Mice on a Budget
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Published on: November 20, 2018

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  • Systematic variation of familiarization and recognition testing times to assess time-of-day effects.
  • Detailed documentation of environmental and experimenter-related factors influencing behavioral outcomes.

    • Main Results:

    • Successful execution of the NOR task during both the mice's active (dark) and inactive (light) periods.
    • Demonstration of a flexible framework adaptable to various experimental conditions, including age, sex, and genetic manipulations.
    • Identification of key parameters crucial for consistent and valid behavioral readouts.

    Conclusions:

    • The developed NOR protocol allows for robust assessment of learning and memory across different circadian phases in mice.
    • This methodology facilitates the study of how sleep-wake cycles and activity patterns modulate cognitive functions.
    • The flexible framework supports diverse research questions in behavioral neuroscience and cognitive research.