Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lagging Strand Synthesis01:59

Lagging Strand Synthesis

60.9K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
60.9K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

16.3K
16.3K
DNA Replication02:40

DNA Replication

58.5K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.5K
Replication in Eukaryotes02:31

Replication in Eukaryotes

203.3K
Overview
203.3K
Replication in Eukaryotes01:29

Replication in Eukaryotes

17.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
17.1K
The Replisome03:01

The Replisome

38.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
38.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mcm10 and RecQL4 Synergize to Activate the Eukaryotic Replicative DNA Helicase.

bioRxiv : the preprint server for biology·2025
Same author

Single-Molecule Imaging Reveals the Mechanism of Bidirectional Replication Initiation in Metazoa.

bioRxiv : the preprint server for biology·2024
Same author

In silico protein interaction screening uncovers DONSON's role in replication initiation.

Science (New York, N.Y.)·2023
Same author

Mechanisms generating cancer genome complexity from a single cell division error.

Science (New York, N.Y.)·2020
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

910

Eukaryotic Lagging Strand Synthesis is Distributive.

Luke Lynch1, Gheorghe Chistol2,3,4,5

  • 1Biochemistry Department, Stanford School of Medicine, Stanford CA94305.

Biorxiv : the Preprint Server for Biology
|September 26, 2025
PubMed
Summary
This summary is machine-generated.

The eukaryotic replisome

More Related Videos

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

7.8K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

Related Experiment Videos

Last Updated: Jan 16, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

910
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

7.8K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA replication requires coordinated synthesis of leading and lagging strands due to DNA's anti-parallel nature.
  • The prevailing model suggests stable tethering of lagging strand polymerases (Pol α/δ) to the replisome for efficient DNA synthesis.

Purpose of the Study:

  • To investigate the dynamic association of DNA Polymerase α (Pol α) and Pol δ with the eukaryotic replisome during DNA replication.
  • To challenge the established model of stable polymerase tethering at replication forks.

Main Methods:

  • Single-molecule imaging of Pol α/δ dynamics at active replication forks.
  • Experiments conducted in Xenopus nuclear extracts to visualize real-time polymerase behavior.

Main Results:

  • Neither Pol α nor Pol δ is stably tethered to the eukaryotic replisome.
  • Lagging strand synthesis occurs distributively, requiring the recruitment of new polymerase molecules for each Okazaki fragment.
  • Evidence for a dynamic, rather than static, mechanism of lagging strand synthesis.

Conclusions:

  • The textbook model of a stably tethered eukaryotic replisome is challenged by these findings.
  • Lagging strand synthesis involves a dynamic recruitment mechanism for DNA polymerases.
  • This study reveals a novel, highly dynamic process for eukaryotic DNA replication.