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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Updated: Jan 16, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Published on: August 9, 2022

Gene expression detection methods in the African turquoise killifish brain.

Emily Whisenant¹, Arne C Lekven¹

¹Department of Biology and Biochemistry, University of Houston, Houston, Texas, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|September 26, 2025

Summary

This summary is machine-generated.

Researchers optimized cost-effective methods for visualizing gene and protein expression in the African turquoise killifish (Nothobranchius furzeri). These new protocols enhance gene expression studies in this emerging model organism.

Keywords:

EZ clear adult fluorescence immunohistochemistry in situ hybridization tissue clearing transcript visualization

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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

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Published on: April 27, 2012

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Last Updated: Jan 16, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

Area of Science:

Neuroscience
Genetics
Developmental Biology

Background:

The African turquoise killifish (Nothobranchius furzeri) is a valuable model for gene expression studies.
Limited cost-effective, high-resolution tools exist for transcript and protein detection in this species.
Analyzing opaque, complex brain tissue requires advanced imaging techniques, but existing methods are time-consuming and equipment-intensive.

Purpose of the Study:

To develop and optimize accessible, high-resolution methods for gene and protein visualization in N. furzeri.
To address limitations in current transcript and protein detection techniques for brain tissue analysis.
To expand the methodological toolkit for N. furzeri research.

Main Methods:

Optimized cryosection-compatible in situ hybridization (ISH) protocols for mRNA detection.
Adapted the EZ-clear method for whole-brain protein visualization.
Validated protocols using Gfap and Dat markers, assessing signal-to-noise ratios and expression patterns.

Main Results:

Achieved high signal-to-noise ratios for colorimetric ISH in thick and thin sections, confirming expected brain region expression.
Successfully adapted EZ-clear for N. furzeri brain tissue clearing, compatible with immunostaining.
Observed potential Gfap upregulation and preserved endogenous fluorescence in transgenic reporter lines.

Conclusions:

Developed cost-effective and accessible protocols for gene and protein visualization in N. furzeri.
Optimized ISH and EZ-clear methods enhance the study of gene expression in this model organism.
These protocols expand the available research tools for N. furzeri.