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A New Approach to Examine Cell-Antibody Avidity with Surface Plasmon Resonance Imaging
Richard B M Schasfoort1,2, Elise van Doorn1, Jos van Weperen2
1Department of Bioengineering Technologies, Technical Medical Centre, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Biosensors
|September 26, 2025
Summary
This study introduces CellVysion, a new instrument for measuring cell-antibody avidity, not just affinity. It quantifies avidity by identifying a "tipping point" in ligand density where cells bind under flow.
Area of Science:
- Biotechnology
- Immunology
- Analytical Chemistry
Background:
- Antibody affinity is commonly measured, but avidity is crucial for antibody design.
- Existing instruments often lack the capability to quantify cell-antibody avidity.
Purpose of the Study:
- To evaluate the novel CellVysion instrument for quantifying cell-antibody avidity.
- To demonstrate the advantages of using a continuous antibody density gradient for avidity assessment.
Main Methods:
- Utilized surface plasmon resonance imaging with a CellVysion instrument.
- Employed a continuous antibody density gradient to determine a
- tipping point
- ligand density.
- Assessed cell binding under defined shear flow conditions.
Main Results:
- Successfully quantified cell-antibody avidity using the CellVysion system.
- Identified a specific ligand density (tipping point) indicative of cell binding stability.
- Demonstrated quantitative avidity assessment across various antibody-cell line combinations.
Conclusions:
- CellVysion offers a novel method for quantitative cell-antibody avidity measurement.
- The tipping point analysis provides critical insights into antibody binding under physiological flow conditions.
- This technique advances antibody design and characterization.
