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Circular RNAs (CircRNAs) regulate gene expression. This study identifies Circ-06958 as a key factor promoting muscle cell proliferation, impacting pig meat quality through a novel regulatory pathway.

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Area of Science:

  • Genetics and Molecular Biology
  • Animal Science
  • Biochemistry

Background:

  • Circular RNAs (CircRNAs) are crucial regulators of gene expression via the competitive endogenous RNA (ceRNA) mechanism.
  • The role of CircRNAs in the development of meat quality in pigs remains largely unexplored.
  • Understanding these mechanisms is vital for improving livestock production and quality.

Purpose of the Study:

  • To characterize CircRNAs in porcine muscles with varying meat quality and myofiber composition.
  • To investigate the function of a specific CircRNA, Circ-06958, in muscle cell proliferation and its regulatory pathway.
  • To elucidate the molecular mechanisms underlying CircRNA's contribution to meat quality formation.

Main Methods:

  • RNA-sequencing (RNA-seq) to identify differentially expressed CircRNAs in porcine longissimus thoracis and semitendinosus muscles.
  • Experimental characterization of Circ-06958, including localization and functional assays in porcine skeletal muscle satellite cells (PMSCs) and C2C12 myoblasts.
  • Construction and validation of the Circ-06958/miR-31-5p/Adenylate Kinase 4 (AK4) regulatory axis using molecular biology techniques.

Main Results:

  • 1126 differentially expressed CircRNAs were identified, with Circ-06958 highly expressed in both studied muscles.
  • Circ-06958, derived from the Long-chain acyl-CoA synthetase 1 (ACSL1) gene, was shown to enhance muscle cell proliferation by promoting cell cycle progression.
  • A novel regulatory axis, Circ-06958/miR-31-5p/AK4, was established, demonstrating that Circ-06958 promotes proliferation via miR-31-5p inhibition and AK4 upregulation.

Conclusions:

  • Circ-06958 plays a significant role in promoting muscle cell proliferation, a key determinant of meat quality.
  • The identified miR-31-5p/AK4 pathway provides novel insights into CircRNA-mediated gene regulation in muscle development.
  • These findings contribute to a deeper understanding of the molecular basis of meat quality in pigs and offer potential targets for genetic improvement.