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Updated: Jan 16, 2026
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Impact of Fabrication Techniques and Polishing Procedures on Surface Roughness of Denture Base Resins
Published on: January 17, 2025
949
Surface Changes Induced by Brushing Increase Candida albicans Biofilms on 3D-Printed Denture Base Resin
Rafaelly Camargo1, Jonatas Silva de Oliveira1, Amanda Costa Ferro1
1Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara 14801-903, São Paulo, Brazil.
Journal of Fungi (Basel, Switzerland)
|September 26, 2025
Summary
Brushing denture resins with toothpaste significantly increases surface roughness and Candida albicans biofilm formation over time. This suggests potential issues for both heat-cured and 3D-printed materials with long-term use.
Area of Science:
- Biomaterials Science
- Dental Materials Science
- Microbiology
Background:
- Long-term effects of cleaning agents on 3D-printed resins are understudied.
- Surface properties and biofilm accumulation are critical for denture hygiene.
Purpose of the Study:
- To assess how different brushing solutions and durations affect surface roughness and Candida albicans biofilm on heat-cured and 3D-printed denture resins.
Main Methods:
- Specimens were brushed simulating 1, 2, and 5 years using water, soap, or toothpaste.
- Surface roughness (Ra) was measured.
- Candida albicans biofilm was quantified via CFU counts, metabolic activity, and confocal microscopy.
Main Results:
- Both resin types showed increased roughness after 2 years, particularly with toothpaste.
- Candida albicans cell counts and metabolic activity increased after 2 years of brushing.
- Increased roughness correlated with enhanced biofilm formation and thickness.
Conclusions:
- Toothpaste significantly roughens both heat-cured and 3D-printed denture resins.
- Long-term brushing with toothpaste may promote Candida albicans biofilm accumulation on these resins.
