Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cross-reactivity

Cross-reactivity

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

Immunoinformatics-Driven Design of a Multiepitope Vaccine for Rustrela Virus-Induced Neurological Diseases: A New Frontier in Encephalitis and Meningoencephalitis Prevention.

Viral immunology·2026

Same author

Decoding the functional landscape of long non-coding RNAs in hepatocellular carcinoma: molecular mechanisms, clinical implications, and therapeutic prospects.

Journal of the Egyptian National Cancer Institute·2026

Same author

Genetic Profiling of Interleukin Variants and their Correlation with Hepatitis C Viral Response to Different Therapeutic Strategies Administered in Pakistani Patients.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research·2026

Same author

Clinical performance of Magmaris bioresorbable scaffold and modern drug-eluting stents (Biomatrix, Ultimaster, Orsiro) in coronary artery disease: a systematic review and meta-analysis.

Acta cardiologica·2026

Same author

Discrimination of Chiral BCPs by Möbius Cyclacene: A DFT Study.

The journal of physical chemistry. A·2026

Same author

Performance of artificial intelligence software: EndoAngel, EndoAID, CAD-EYE, GI Genius, and EndoScreener in adenoma detection: an extended network meta-analysis.

European journal of gastroenterology & hepatology·2026

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

Immunogenic Response Assessment of Hepatitis Delta Virus Antigen from Pakistani Isolate in Rabbits Using a

Hifza Manzoor¹, Muhammad Shahid¹, Nadeem Ahmed²

¹Division of Molecular Virology and Infectious Diseases, Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.

Viral Immunology

|September 26, 2025

Summary

Researchers developed a new method to produce Hepatitis Delta Virus (HDV) antigens and antibodies. This advancement aims to improve diagnostic tests and potential vaccines for HDV infection, which currently lacks specific treatments.

Keywords:

hepatitis delta virus prokaryotic expression system protein purification rabbit immunization

More Related Videos

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Published on: November 22, 2021

Related Experiment Videos

Last Updated: Jul 18, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Published on: January 20, 2019

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Published on: November 22, 2021

Area of Science:

Virology
Immunology
Biotechnology

Background:

Hepatitis Delta Virus (HDV) is a defective virus requiring Hepatitis B Virus (HBV) for replication, posing significant diagnostic and therapeutic challenges.
Current diagnostic assays for HDV show variability due to a lack of standardization and unavailability of specific antibodies.
There is an urgent need for reliable diagnostic tools and potential vaccines against HDV, especially for locally predominant genotypes.

Purpose of the Study:

To produce a recombinant Hepatitis Delta Antigen (HDAg) protein from a local HDV genotype I isolate.
To generate specific anti-HDAg antibodies in rabbits using the purified recombinant HDAg.
To lay the groundwork for developing standardized in-house diagnostic assays and potential vaccines for HDV.

Main Methods:

The antigenic region of HDV HDAg (genotype I) was amplified, cloned into a pET-28a vector, and expressed in Escherichia coli (E. coli).
Recombinant HDAg protein with a C-terminal His-tag was purified using affinity chromatography and confirmed by SDS-PAGE and Western blot.
Japanese White rabbits were immunized with purified HDAg using a specific protocol, and anti-HDAg antibody levels were quantified using ELISA.

Main Results:

Recombinant HDAg protein was successfully expressed and purified to over 90% purity in E. coli.
Specific anti-HDAg antibodies were successfully produced in immunized rabbits.
The developed antibodies showed potential for use in diagnostic assays.

Conclusions:

The successful expression and purification of recombinant HDAg and subsequent antibody production represent a significant step towards overcoming diagnostic limitations for HDV.
These antibodies can be utilized for developing standardized, in-house diagnostic assays for HDV genotype I.
This work may contribute to reducing reliance on imported diagnostic reagents and developing targeted vaccines against prevalent HDV genotypes.