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Published on: July 21, 2018
Hsa_circ_0075451 promotes NSCLC proliferation, metastasis, and glycolysis through interaction with the RNA-binding
ChongYu Su1, YuXuan Wang1, Kang Shi1
1Department of Thoracic Surgery II, Beijing Chest Hospital, Capital Medical University, District 1, No.9 Beiguan Street, Tongzhou District, Beijing City, 101149, China.
Objective:
To investigate the mechanism of hsa_circ_0075451 in promoting NSCLC proliferation, metastasis, and glycolysis through interaction with RBM4.
Methods:
Cancerous tissues and paracancerous tissues were collected from 53 cases of NSCLC. RT-qPCR or Western blot was performed to detect hsa_circ_0075451 and RBM4. Relevant sequences or plasmids were transfected into NSCLC cell line A549, and the malignant phenotype of NSCLC cells was detected by CCK-8, EdU, AnnexinV-PI double staining assay, and Transwell. Glycolysis was assessed by glucose consumption, lactic acid production, and glycolysis-related proteins. In vivo tumorigenesis assays were performed to investigate hsa_circ_0075451 functions in NSCLC. Mechanistic studies were analyzed to verify the interaction of hsa_circ_0075451 and RBM4.
Results:
Hsa_circ_0075451 levels were elevated in NSCLC, and reducing its expression diminished cell proliferation, metastasis, glycolysis, and tumor expansion. Hsa_circ_0075451 interacted with RBM4. RBM4 down-regulation restricted NSCLC cell proliferation, metastasis, and glycolysis. RBM4 up-regulation opposed the effects of hsa_circ_0075451 knockdown.
Conclusion:
Hsa_circ_0075451 promotes NSCLC proliferation, metastasis and glycolysis by interacting with RBM4.
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