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Morphogenesis of respiratory syncytial virus in a green monkey kidney cell line (Vero)
Abstract:
The structure and morphogenesis of respiratory syncytial (RS) virus particles in a green monkey kidney cell line (Vero) were examined. Infected cells contained dense intracytoplasmic inclusions composed of filamentous structures. In places where inclusion material was associated with membranes, structural modifications were induced. There was a thickening of the membrane and an addition of projections 12 to 15 nm in length. The same changes were most frequently observed after association of isolated filamentous structures with the cytoplasmic membrane. The budding-off process was clearly visualized. The diameter of mature virus particles varied between 90 and 130 nm and that of the internal component varied between 11 and 15 nm. The similarities between ultrastructural features of cells infected with RS virus and pneumonia virus of mice are pointed out. It is proposed that these two viruses should be classified together in a third subgroup of myxoviruses.
Insights
Researchers studied respiratory syncytial (RS) virus structure and development in Vero cells. They observed viral filaments inducing membrane changes and budding, revealing particle dimensions and suggesting a new myxovirus classification.
Area of Science:
- Virology
- Cell Biology
- Microbiology
Background:
- Respiratory syncytial virus (RSV) is a significant human pathogen.
- Understanding RSV particle morphogenesis is crucial for antiviral development.
- Previous studies lacked detailed ultrastructural analysis of RSV replication.
Purpose of the Study:
- To investigate the structure and morphogenesis of respiratory syncytial virus (RSV) particles.
- To examine the effects of RSV infection on host cell membranes.
- To compare RSV ultrastructure with other myxoviruses for classification purposes.
Main Methods:
- Infected green monkey kidney (Vero) cells were examined using electron microscopy.
- Intracytoplasmic inclusions and membrane-associated viral structures were analyzed.
- Morphometric analysis of mature virus particles and internal components was performed.
Main Results:
- Dense, filamentous intracytoplasmic inclusions were observed in infected cells.
- RSV association with cellular membranes induced thickening and projection formation (12-15 nm).
- Virus budding was visualized; mature particles measured 90-130 nm, internal components 11-15 nm.
Conclusions:
- RSV morphogenesis involves significant host cell membrane modifications.
- Ultrastructural similarities exist between RSV and pneumonia virus of mice.
- A proposal is made to classify RSV and pneumonia virus of mice together in a new myxovirus subgroup.