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HpBoRB, a helminth-derived CCP domain protein which binds RELMβ.
Vivien Shek1, Abhishek Jamwal2, Danielle J Smyth1
1Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee, the United Kingdom of Great Britain and Northern Ireland.
International Journal for Parasitology
|September 26, 2025
Summary
The intestinal nematode Heligmosomoides polygyrus bakeri (Hpb) secretes proteins that interact with host immunity. A newly identified Hpb protein, HpBoRB, binds to the anti-helminth protein resistin-like molecule beta (RELMβ), potentially evading host defenses.
Area of Science:
- Parasitology
- Immunology
- Molecular Biology
Background:
- Helminth infections establish by releasing immunomodulatory proteins to evade host immunity.
- The intestinal nematode Heligmosomoides polygyrus bakeri (Hpb) secretes numerous immunomodulatory proteins, many containing Complement Control Protein (CCP) domains.
Purpose of the Study:
- To identify novel Hpb-secreted proteins with immunomodulatory functions.
- To investigate potential interactions between Hpb proteins and host immune molecules.
Main Methods:
- Genome-wide identification of CCP domain-containing proteins in Hpb.
- Screening of cloned Hpb proteins for interactions with host immune proteins using Avidity-based Extracellular Interaction Screening (AVEXIS).
- Validation of interactions using ELISA, competition assays, size exclusion chromatography, and surface plasmon resonance.
Main Results:
- Confirmed known interactions between Hpb proteins (HpBARI, TGM1) and their targets (ST2, TGFBR2).
- Discovered a novel interaction between a 2 CCP domain Hpb protein, named HpBoRB, and mouse resistin-like molecule beta (RELMβ).
- HpBoRB exhibits specific, heat-labile binding to RELMβ with subnanomolar affinity.
Conclusions:
- Hpb secretes a diverse repertoire of CCP domain proteins, including novel interactors with host immune molecules.
- The newly identified HpBoRB protein may interfere with the anti-helminth function of RELMβ, contributing to parasite persistence.
- This study expands the understanding of host-parasite interactions mediated by secreted Hpb proteins.
Keywords:
AVEXISComplement Control ProteinHeligmosomoides polygyrus bakeriImmunomodulationProtein–protein interactionRELMβMore Related Videos
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