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SeXTH23 enhances salt tolerance by regulating succulence and osmotic adjustment in aspen hybrid
Huirong Duan1, Richard John Tiika2, Guangxin Cui1
1Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Abstract:
Salicornia europaea serves as a key halophyte model for studying salt adaptation due to its remarkable ability to thrive under high salinity. In the present study, 50-300 mM NaCl enhanced S. europaea growth, accompanied with increased tissue succulence and water content, also effective ion accumulation and distribution. SeXTH23, harboring a conserved catalytic domain and secretory signal peptide, was highly expressed in shoots and vascular tissues, indicating its potential role in vascular cell wall remodeling during stress. Overexpression of SeXTH23 in transgenic aspen hybrid improved salt tolerance by enhancing succulence, water content, proline accumulation and photosynthetic efficiency, while reducing oxidative damage (lower MDA) and water loss rate. SeXTH23-RNAi S. europaea plants exhibited reduced dry weight, succulence, and rapid water lost. These results form a foundation for the application of SeXTH23 as genetic target for engineering salt-tolerant crops.
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