MMP13-Expressing and COL11A1-Expressing Cancer-Associated Fibroblasts: Key Drivers of Esophageal Squamous Cell...

The tumor microenvironment comprises various cell types, and cancer-associated fibroblasts (CAFs) are crucial contributors to cancer progression and metastasis. CAFs also play an important role in esophageal squamous cell carcinoma and have been extensively studied in this context. However, the association between CAFs and progression across pathological stages has not yet been reported. To identify these specific CAFs, we used a case-oriented approach for single-cell RNA sequencing. Consequently, we identified 3 CAF clusters classified as myofibroblastic CAFs (myCAFs), which increased in number as the cancer progressed. Pathway analysis revealed that the 3 CAF clusters had distinct properties. These CAFs were named MMP13⁺, COL11A1⁺, and SFRP4⁺ myCAFs based on their characteristic gene expression. We also investigated the distribution of various immune cells within the tumor microenvironment associated with the 3 different CAF clusters. The results revealed the presence of different types of immune cells, including M2 macrophages, regulatory T cells, and interferon gamma⁺ programmed death-1⁺ T cells and interferon gamma⁺ programmed death-1^- T cells. Next, we evaluated the presence of these 3 CAF subtypes in surgically resected specimens from patients with advanced esophageal squamous cell carcinoma using RNA in situ hybridization. Analysis of the association between these 3 CAF subtypes and prognosis showed that 2 subtypes (MMP13⁺ and COL11A1⁺ myCAFs) were associated with poor prognosis. MMP13⁺ myCAFs were associated with poorly differentiated infiltration patterns, whereas COL11A1⁺ myCAFs were associated with lymph node metastasis. These results suggest that future treatments targeting these CAFs and patient stratification based on these CAFs are warranted.