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Related Experiment Video

Updated: Jan 16, 2026

Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Determination of Self- and Inter-incompatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Identification of Self-Incompatibility Related Genes in Sweet Cherry Based on Transcriptomic Analysis.

Chen Feng^1,2,3, Chuanbao Wu^1,2,3, Jing Wang^1,2,3

¹Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China.

|September 27, 2025

Summary

Sweet cherry self-incompatibility (GSI) reduces fruit set. This study identified key genes and pathways in

Keywords:

Co-expression analysis gene expression pattern self-incompatible mechanism transcription factor ‘Tieton’

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Published on: June 30, 2023

Area of Science:

Plant reproductive biology
Molecular genetics
Agricultural science

Background:

Most sweet cherry varieties have gametophytic self-incompatibility (GSI), requiring cross-pollination for fruit production.
This necessitates complex orchard management and increases costs.
Developing self-compatible cultivars is crucial for efficient sweet cherry cultivation.

Purpose of the Study:

To investigate the molecular mechanisms of GSI in sweet cherries.
To identify key genes and pathways involved in the self-incompatibility response.
To provide genetic resources for breeding self-compatible sweet cherry varieties.

Main Methods:

Assessed fruit set rates of 'Tieton' under self- and cross-pollination.
Performed transcriptome analysis of 'Tieton' styles at various time points post-pollination.
Utilized KEGG pathway analysis and co-expression analysis to identify key genes and transcription factors.

Main Results:

'Tieton' showed significantly lower self-fruit set compared to cross-pollination.
Identified 8148 differentially expressed genes (DEGs).
Key pathways involved in GSI include plant-pathogen interaction, hormone signal transduction, and MAPK signaling.
Identified 13 core transcription factors and 132 associated core DEGs.

Conclusions:

This study provides initial insights into the sweet cherry GSI molecular network.
Identified crucial genes and transcription factors regulating self-incompatibility.
Offers a theoretical foundation and genetic resources for breeding self-compatible sweet cherries.