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Study of FOXL2 Regulation on Ovarian Function in Chlamys farreri Through Comparative ChIP-Seq and Transcriptome

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  • 1College of life Science, Yantai University, Yantai 264006, China.

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Summary

The study reveals forkhead box protein L2 (FOXL2) regulates scallop oogenesis by influencing genes involved in sex development and hormone synthesis. Knockdown of FOXL2 impacts ovarian function and gene expression pathways critical for gonad development.

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Area of Science:

  • Marine biology
  • Developmental biology
  • Molecular genetics

Background:

  • The transcription factor FOXL2 (forkhead box protein L2) is crucial for mammalian ovarian development, but its role in marine invertebrates remains understudied.
  • Previous experiments showed FOXL2 knockdown in C. farreri disrupted oogenesis and induced spermatogonial-like cells.

Purpose of the Study:

  • To comprehensively analyze the regulatory pathways and key genes controlled by FOXL2 during oogenesis in the scallop C. farreri.
  • To investigate the molecular mechanisms underlying FOXL2's function in gonad development using transcriptomic and ChIP-seq data.

Main Methods:

  • Transcriptome sequencing and differential gene expression analysis were performed on ovarian tissues of C. farreri after FOXL2 knockdown via RNA interference (RNAi).
  • Chromatin Immunoprecipitation Sequencing (ChIP-seq) was employed using an antibody against recombinant C. farreri FOXL2 protein to identify direct targets.

Main Results:

  • FOXL2 knockdown resulted in 389 upregulated and 1615 downregulated genes in C. farreri ovaries.
  • Differentially expressed genes were significantly enriched in pathways related to steroid hormone synthesis, spermatogenesis, gonad development, and ovarian maintenance, including FoxO and estrogen signaling.
  • ChIP-seq identified direct FOXL2 regulation of key genes involved in sex gonad development, such as Wnt4, SIRT1, KRAS, and NOTCH1.

Conclusions:

  • FOXL2 plays a critical role in regulating oogenesis and gonad development in scallops.
  • This research elucidates FOXL2's molecular regulatory network in C. farreri oogenesis, providing foundational insights for understanding its function as a transcription factor in marine invertebrates.