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Active Peptides from Crayfish Shell: Isolation, Purification, Identification and Cytoprotective Function on Cells

Chan Bai1, Wenqing Wang1,2, Guowei Huang1,3

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Crayfish shell peptides show strong antioxidant and ACE inhibitory effects. These bioactive peptides may serve as natural ingredients for health-promoting antihypertensive and antioxidant agents.

Keywords:
biological activitycrayfish shell peptidecytoprotective effectmass spectrometry identification

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Area of Science:

  • Biochemistry
  • Food Science
  • Pharmacology

Background:

  • Crayfish shells are a rich source of proteins with potential bioactive properties.
  • Developing functional ingredients from seafood byproducts addresses waste valorization and health promotion.

Purpose of the Study:

  • To develop and characterize crayfish shell peptides with enhanced antioxidant and angiotensin-I-converting enzyme (ACE) inhibitory activities.
  • To investigate the potential of these peptides as antihypertensive and antioxidant agents.

Main Methods:

  • Crayfish shell protein hydrolysates (CSPH) were prepared and fractionated by molecular weight.
  • Antioxidant activity was assessed using DPPH and ABTS radical scavenging assays.
  • ACE inhibitory activity was measured, and active peptides were identified using LC-MS/MS.
  • Cellular assays (HUVECs, HepG2) were used to evaluate nitric oxide (NO), endothelin-1 (ET-1), and reactive oxygen species (ROS) inhibition, and protection against oxidative damage.

Main Results:

  • CSPH2 (3-5 kDa) demonstrated significant antioxidant and ACE inhibitory activities.
  • Purified antioxidant (APAPLPPPAP) and ACE inhibitory (QGPDDPLIPIM) peptides were identified.
  • These peptides increased NO, decreased ET-1, inhibited ROS in HUVECs, and protected HepG2 cells from oxidative stress.

Conclusions:

  • Crayfish shell peptides possess potent antioxidant and ACE inhibitory properties.
  • These peptides demonstrate potential for use as functional ingredients in antihypertensive and antioxidant applications.
  • This study highlights the health benefits and utilization of crayfish processing byproducts.