Active Peptides from Crayfish Shell: Isolation, Purification, Identification and Cytoprotective Function on Cells...

Area of Science:

• Biochemistry
• Food Science
• Pharmacology

Background:

• Crayfish shells are a rich source of proteins with potential bioactive properties.
• Developing functional ingredients from seafood byproducts addresses waste valorization and health promotion.

Purpose of the Study:

• To develop and characterize crayfish shell peptides with enhanced antioxidant and angiotensin-I-converting enzyme (ACE) inhibitory activities.
• To investigate the potential of these peptides as antihypertensive and antioxidant agents.

Main Methods:

• Crayfish shell protein hydrolysates (CSPH) were prepared and fractionated by molecular weight.
• Antioxidant activity was assessed using DPPH and ABTS radical scavenging assays.
• ACE inhibitory activity was measured, and active peptides were identified using LC-MS/MS.
• Cellular assays (HUVECs, HepG2) were used to evaluate nitric oxide (NO), endothelin-1 (ET-1), and reactive oxygen species (ROS) inhibition, and protection against oxidative damage.

Main Results:

• CSPH2 (3-5 kDa) demonstrated significant antioxidant and ACE inhibitory activities.
• Purified antioxidant (APAPLPPPAP) and ACE inhibitory (QGPDDPLIPIM) peptides were identified.
• These peptides increased NO, decreased ET-1, inhibited ROS in HUVECs, and protected HepG2 cells from oxidative stress.

Conclusions:

• Crayfish shell peptides possess potent antioxidant and ACE inhibitory properties.
• These peptides demonstrate potential for use as functional ingredients in antihypertensive and antioxidant applications.
• This study highlights the health benefits and utilization of crayfish processing byproducts.