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Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features
Anna Igorevna Alekseeva1, Alexandra Vladislavovna Sentyabreva1,2, Vera Vladimirovna Kudelkina1
1Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution 'Petrovsky National Research Center of Surgery', 117418 Moscow, Russia.
International Journal of Molecular Sciences
|September 27, 2025
Summary
This study characterizes a rat glioma model (glioma101.8) for brain tumor research. The model exhibits genetic and cellular similarities to human gliomas, aiding in personalized therapy development.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Animal Models
Background:
- Developing accurate models for central nervous system (CNS) tumors is crucial for personalized therapy.
- Existing human biomaterial models have limitations in data generation.
- Recent CNS tumor classification changes necessitate models with morphological and genetic similarity to human tumors.
Purpose of the Study:
- To characterize a chemically induced, tissue-transplantable rat glioma model (glioma101.8).
- To evaluate its suitability for studying glioma carcinogenesis and developing personalized therapies.
- To identify key genetic aberrations and cellular compositions relevant to human gliomas.
Main Methods:
- Magnetic Resonance Imaging (MRI)
- Immunohistochemistry (IHC)
- Single-cell RNA sequencing (scRNA-seq)
- Quantitative Polymerase Chain Reaction (qPCR-RT)
Main Results:
- Detailed characterization of the rat glioma 101.8 strain.
- Determination of its cellular composition and microenvironment.
- Identification of major genetic signatures within the glioma model.
Conclusions:
- The rat glioma 101.8 strain serves as a valuable, representative model for CNS tumors.
- This model can aid in identifying novel signaling pathways for oncogenesis.
- It holds potential for advancing personalized therapeutic strategies in neuro-oncology.
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