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Purified Native Collagen Extracellular Matrix Plus Polyhexamethylene Biguanide Functions as a Barrier to Protect
Rami A Nasrallah1, Kelly A Kimmerling2, James L Cook3,4
1Organogenesis Innovation Center, San Diego, CA 92121, USA.
International Journal of Molecular Sciences
|September 27, 2025
Summary
Purified native collagen extracellular matrix plus polyhexamethylene biguanide (PCMP) improved complex wound healing in canines. PCMP reduced bacterial load and supported wound repair, suggesting enhanced patient outcomes.
Area of Science:
- Biomaterials Science
- Wound Healing Research
- Veterinary Surgery
Background:
- Complex surgical wounds often require repeated irrigation and debridement (I&D), posing significant patient burdens.
- Purified native collagen extracellular matrix plus polyhexamethylene biguanide (PCMP) acts as an antimicrobial barrier, potentially aiding innate wound healing.
- Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in complex wound infections.
Purpose of the Study:
- To evaluate the efficacy of PCMP in protecting an in vivo complex wound model.
- To assess PCMP's impact on clinical healing, microbial load, implant stability, and host gene expression.
- To determine if PCMP can limit bioburden and biofilm reformation in challenging wound environments.
Main Methods:
- Canine subjects with bilateral fibular defects stabilized with MRSA-contaminated implants underwent I&D.
- Wounds were treated with either PCMP or non-adherent pads.
- Outcome assessments included radiography, wound scoring, microbial culture, histology, and gene expression analysis.
Main Results:
- PCMP application significantly improved clinical wound healing scores at days 3 and 7.
- Histological analysis showed a trend towards improved wound repair with PCMP treatment.
- Radiography confirmed implant stability, and microbial assessments showed a minor reduction in bacterial load (0.5 log fold-change).
- Gene expression analysis revealed significant upregulation of matrix metalloproteinases and immune-modulating cytokines.
Conclusions:
- PCMP application demonstrated improved wound scores, reduced bacterial load, and significant upregulation of key gene expression pathways compared to controls.
- PCMP effectively limited bioburden and biofilm reformation, supporting wound progression in a challenging environment.
- These findings suggest PCMP has the potential to enhance outcomes for patients with complex wounds.
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