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Published on: June 13, 2014
Novel Organomineral Complex with Prolonged Antitumor Action
Olga Ilinskaya1, Galina Yakovleva1, Pavel Zelenikhin1
1Microbiology Department, Institute of Fundamental Medicine and Biology of Kazan (Volga-Region) Federal University, Kazan Federal University, Kremlevskaya St., 18, Kazan 420008, Russia.
Researchers developed a novel drug delivery system using binase, an antitumor enzyme, immobilized on a rock carrier for sustained release in the gastrointestinal tract. This approach enhances cancer cell targeting and offers potential for long-acting antitumor therapy.
Area of Science:
- Biotechnology
- Materials Science
- Oncology
Background:
- Cancer cell proliferation is often targeted by blocking the MAPK pathway.
- Drug resistance and KRAS mutations limit the effectiveness of current cancer therapies.
- Ribonuclease binase from Bacillus pumilus interacts with EGFR and RAS, inhibiting this pathway.
Purpose of the Study:
- To design, optimize, and characterize a novel complex for prolonged release of antitumor binase.
- To immobilize binase on microgranular clinoptilolite-containing rock for sustained gastrointestinal release.
- To evaluate the antitumor and detoxifying properties of the developed complex.
Main Methods:
- Characterization of clinoptilolite carrier using electron microscopy (TEM, SEM) and computed tomography.
- Measurement of binase particle size via atomic force microscopy.
- Assessment of binase stability and activity using calorimetry and model gastrointestinal fluids.
Main Results:
- The carrier's granularity, porosity, and elemental composition were analyzed.
- Binase particles (7 nm) effectively penetrated the carrier's pores.
- Binase demonstrated stability at high temperatures and retained activity in simulated GI fluids.
- The complex exhibited gradual enzyme release over 20 hours, showing cytotoxicity against adenocarcinoma cells.
Conclusions:
- A novel long-acting complex of antitumor binase immobilized on clinoptilolite was successfully created.
- The complex demonstrates sustained release and potent cytotoxicity against cancer cells.
- This system offers a promising approach for long-acting antitumor and detoxifying therapy.
