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Spermatogenesis01:22

Spermatogenesis

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Nor1 and Mitophagy: An Insight into Sertoli Cell Function Regulating Spermatogenesis Using a Transgenic Rat Model.

Bhola Shankar Pradhan1,2, Deepyaman Das3,4, Hironmoy Sarkar3

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|September 27, 2025
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Summary

Neuron-derived orphan receptor 1 (Nor1) is crucial for male fertility. Its knockdown in Sertoli cells impairs sperm development and reduces fertility, highlighting its role in regulating male reproductive health.

Keywords:
Nor1Sertoli cellgerm cellmale fertilitymitochondriamitophagy

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Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Cellular Biology

Background:

  • Male infertility affects global health, with many idiopathic cases.
  • Sertoli cells (Scs) are vital for germ cell (Gc) development and male fertility.
  • The role of mitophagy-related genes in Sc differentiation and male fertility remains unclear.

Purpose of the Study:

  • To investigate the role of mitophagy-related genes in Sc differentiation.
  • To identify novel genes regulating male fertility through Sc function.
  • To explore the function of Neuron-derived orphan receptor 1 (Nor1) in male reproduction.

Main Methods:

  • Analysis of microarray dataset GSE48795 to identify mitophagy-related hub genes.
  • Generation of a transgenic rat model with Sc-specific knockdown of Nor1 during puberty.
  • Functional analysis of spermatogenesis and fertility in transgenic rats.

Main Results:

  • Identified 12 mitophagy-related hub genes, including Bcl2 and Fos.
  • Nor1 was identified as a potential mitophagy-related gene associated with Sc maturation.
  • Sc-specific knockdown of Nor1 resulted in impaired spermatogenesis and reduced male fertility.

Conclusions:

  • Nor1 plays a significant role in regulating Sertoli cell function.
  • Nor1 is a key mitophagy-related gene essential for maintaining male fertility.
  • Targeting Nor1 may offer therapeutic strategies for male infertility.