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Biofilms Exposed: Innovative Imaging and Therapeutic Platforms for Persistent Infections
Manasi Haval1, Chandrashekhar Unakal2, Shridhar C Ghagane3
1Department of Pre-Clinical Research and Drug Development, Cytxon Biosolutions Pvt. Ltd., Hubballi 580031, Karnataka, India.
Antibiotics (Basel, Switzerland)
|September 27, 2025
Summary
Biofilms resist treatment due to complex mechanisms. This review explores advanced technologies and novel therapies, like synthetic biology, for effective biofilm management and infection control.
Area of Science:
- Microbiology
- Infectious Diseases
- Biotechnology
Background:
- Biofilms present significant challenges in treating infectious diseases due to their resistance to conventional therapies.
- Their resilience stems from extracellular polymeric substances, metabolic dormancy, and quorum sensing, leading to chronic infections and economic burdens.
Purpose of the Study:
- To review the molecular and structural basis of biofilm persistence.
- To critically assess limitations of current diagnostic and therapeutic strategies.
- To highlight emerging technologies and interventions for biofilm management.
Main Methods:
- Examination of molecular and structural complexities driving biofilm persistence.
- Review of advanced technologies like super-resolution microscopy, microfluidics, and AI-driven modeling.
- Analysis of novel therapeutic approaches including CRISPR-Cas bacteriophages, quorum-sensing antagonists, nanocarriers, and synthetic biology interventions.
Main Results:
- Advanced technologies are enhancing the understanding of biofilm dynamics and heterogeneity.
- Novel therapies show promise in targeting biofilms specifically.
- In vivo and ex vivo models are improving translational applicability of research findings.
Conclusions:
- A paradigm shift is occurring from reactive antibiotic therapy to precision-guided biofilm management.
- Integrating cutting-edge technologies and systems biology offers a comprehensive framework for disrupting biofilms.
- Emerging interventions promise to redefine infection treatment in the post-antibiotic era.
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