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Hsp101-1 Orchestrates Thermotolerance in Rice via Pre-Activated Transcriptional Networks and Modular Cross-Tissue
Hang Yu1,2,3,4, Liqun Jiang1,2,3,4, Bingrui Sun1,2,3,4
1Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Genes
|September 27, 2025
Summary
Overexpressing heat shock protein 101-1 (Hsp101-1) in rice enhances thermotolerance by stabilizing gene networks and coordinating responses to heat stress, crucial for climate resilience.
Area of Science:
- Plant Science
- Molecular Biology
- Genetics
Background:
- Rice production is threatened by increasing global temperatures.
- Developing heat-tolerant rice varieties is essential for food security.
- Heat shock proteins play a critical role in plant stress responses.
Purpose of the Study:
- To characterize transcriptomic dynamics in rice under heat stress.
- To identify gene regulatory modules associated with Hsp101-1.
- To understand how Hsp101-1 contributes to thermotolerance.
Main Methods:
- Transcriptomic analysis of wild-type and Hsp101-1 overexpressing rice lines.
- Weighted gene co-expression network analysis (WGCNA) on flag leaves and spikelets.
- Analysis across six time points (0-24 hours) at 40°C.
Main Results:
- Hsp101-1 overexpression significantly reduced differentially expressed genes (DEGs) under heat stress.
- WGCNA identified key gene modules (MEdarkslateblue, MEturquoise, MElightpink4) correlated with Hsp101-1 activity.
- 71 shared module genes were upregulated in Hsp101-1 overexpressing lines, indicating activation of protective targets.
Conclusions:
- Hsp101-1 enhances rice thermotolerance by pre-stabilizing transcriptomic networks.
- It reduces transcriptional fluctuations and coordinates tissue-specific responses to heat stress.
- This provides a framework for developing climate-resilient rice varieties.
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