Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of blastocyst morphokinetics assessed by time-lapse technology and its correlation with human IVF treatment outcome.

Physiology international·2026
Same author

Successful establishment of chicken primordial germ cell (PGC) lines from the endangered Slovakian indigenous chicken breed: the Oravka.

Poultry science·2026
Same author

X-Linked EGFP Reporter as a Tool to Examine X-Chromosome Inactivation in Mouse Embryos and Embryonic Fibroblasts.

Biomolecules·2026
Same author

Detection of Mycotoxins in Fallow Deer Milk and Feces: Evidence of Climate-Driven Contamination in a Comparative Study of Two Weather-Divergent Years in Hungary.

Toxins·2026
Same author

In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.

Biology·2025
Same author

[The association of COVID-19 pandemic misconceptions with mental health and quality of life among recreational e-sports players].

Orvosi hetilap·2025

Related Experiment Video

Updated: Jan 16, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

5.8K

Detection of Development-Specific MicroRNAs in Rabbit Embryos and Culture Media: A Potential Biomarker Approach for

María Salinas1,2, Nikolett Tokodyné Szabadi1,2, Gréta Dévai1,2

  • 1Institute of Genetics and Biotechnology, Animal Bio-Technology Department, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, Hungary.

Genes
|September 27, 2025
PubMed
Summary
This summary is machine-generated.

Researchers identified specific microRNAs (miRNAs) in rabbit embryo culture medium that indicate embryo quality and developmental stage. This discovery enables a non-invasive assay for predicting pregnancy outcomes in humans.

Keywords:
IVFbiomarkerblastocystembryo viabilitymicroRNA

More Related Videos

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

13.3K
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
07:36

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Published on: August 27, 2020

6.7K

Related Experiment Videos

Last Updated: Jan 16, 2026

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

5.8K
Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
07:02

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Published on: May 16, 2019

13.3K
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
07:36

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Published on: August 27, 2020

6.7K

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression and crucial in biological processes.
  • Rabbit embryos serve as a model to identify biomarkers for human embryo development.
  • Investigating embryo culture medium for secreted miRNAs offers a non-invasive approach.

Purpose of the Study:

  • To evaluate seven specific microRNAs (miRNAs) as potential biomarkers for rabbit embryo developmental quality.
  • To correlate miRNA expression levels in embryos and culture media with embryo developmental stage (day 4 vs. day 6).
  • To develop a non-invasive method for assessing embryo viability and predicting pregnancy outcomes.

Main Methods:

  • Analysis of seven specific miRNAs (miR-24-3p, miR-28-3p, miR-103a-3p, miR-181a-5p, miR-191-5p, miR-320a-3p, miR-378a-3p) in 4- and 6-day-old rabbit embryos and their culture media.
  • Correlation of miRNA expression profiles with embryo quality, size, and developmental status.
  • Assessment of miRNA detectability in spent culture medium without prior RNA isolation.

Main Results:

  • Significant differences in miRNA expression were observed between 4-day-old and 6-day-old rabbit embryos.
  • While less variation was seen in culture media between age groups, analysis of individual culture media identified lower-quality embryos.
  • Secreted miRNAs in culture medium correlate with embryo quality, reflecting developmental milestones.

Conclusions:

  • Detected miRNAs in embryo culture medium can reliably indicate progression to the blastocyst stage.
  • A refined panel of miRNAs is essential for developing implantation-competent embryos in rabbits and humans.
  • A non-invasive assay using secreted miRNAs can predict implantation and pregnancy success, benefiting human reproductive medicine.